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crispr single grna design tool  (Synthego Inc)

 
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    Synthego Inc crispr single grna design tool
    Screening and validation of <t>CRISPR/Cas9-mediated</t> STAR KO in MA-10 cells. ( A ) Western blot screening for STAR KO cell line following CRISPR/Cas9-mediated STAR KO and FACS. Samples in lane 5 (STARKO2) and lane 8 (STARKO1) show no STAR band and, therefore, are STAR KOs. ( B ) qRT-PCR analyses of STARKO1 and STARKO2 where Gapdh was used as the housekeeping gene. STAR 1, STAR 2, STAR 3, and STAR 4 are primers for various regions of the mouse Star gene. Data are shown as the mean ± SD ( n = 3). ** p < 0.01; *** p < 0.001. ( C ) BLAST DNA sequence of Star in STARKO1 cells (top) compared to wild-type mouse Star (bottom). Dashes represent nucleotide deletions. Amino acid sequence of wild-type STAR (top) compared to that of STARKO1 (bottom). Highlighted amino acids represent sequence changes. ( D ) BLAST DNA sequence of Star in STARKO2 cells (top) compared to wild-type mouse Star (bottom). Dashes represent nucleotide deletions. Amino acid sequence of wild-type STAR (top) compared to that of STARKO2 (bottom). Underlined amino acids represent sequence changes.
    Crispr Single Grna Design Tool, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crispr+single+grna+design+tool/crispr+single+grna+design+tool/pmc07922663-169-16-22
    Average 90 stars, based on 1 article reviews
    crispr single grna design tool - by Bioz Stars, 2026-09
    90/100 stars

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    1) Product Images from "Role of Constitutive STAR in Leydig Cells"

    Article Title: Role of Constitutive STAR in Leydig Cells

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms22042021

    Screening and validation of CRISPR/Cas9-mediated STAR KO in MA-10 cells. ( A ) Western blot screening for STAR KO cell line following CRISPR/Cas9-mediated STAR KO and FACS. Samples in lane 5 (STARKO2) and lane 8 (STARKO1) show no STAR band and, therefore, are STAR KOs. ( B ) qRT-PCR analyses of STARKO1 and STARKO2 where Gapdh was used as the housekeeping gene. STAR 1, STAR 2, STAR 3, and STAR 4 are primers for various regions of the mouse Star gene. Data are shown as the mean ± SD ( n = 3). ** p < 0.01; *** p < 0.001. ( C ) BLAST DNA sequence of Star in STARKO1 cells (top) compared to wild-type mouse Star (bottom). Dashes represent nucleotide deletions. Amino acid sequence of wild-type STAR (top) compared to that of STARKO1 (bottom). Highlighted amino acids represent sequence changes. ( D ) BLAST DNA sequence of Star in STARKO2 cells (top) compared to wild-type mouse Star (bottom). Dashes represent nucleotide deletions. Amino acid sequence of wild-type STAR (top) compared to that of STARKO2 (bottom). Underlined amino acids represent sequence changes.
    Figure Legend Snippet: Screening and validation of CRISPR/Cas9-mediated STAR KO in MA-10 cells. ( A ) Western blot screening for STAR KO cell line following CRISPR/Cas9-mediated STAR KO and FACS. Samples in lane 5 (STARKO2) and lane 8 (STARKO1) show no STAR band and, therefore, are STAR KOs. ( B ) qRT-PCR analyses of STARKO1 and STARKO2 where Gapdh was used as the housekeeping gene. STAR 1, STAR 2, STAR 3, and STAR 4 are primers for various regions of the mouse Star gene. Data are shown as the mean ± SD ( n = 3). ** p < 0.01; *** p < 0.001. ( C ) BLAST DNA sequence of Star in STARKO1 cells (top) compared to wild-type mouse Star (bottom). Dashes represent nucleotide deletions. Amino acid sequence of wild-type STAR (top) compared to that of STARKO1 (bottom). Highlighted amino acids represent sequence changes. ( D ) BLAST DNA sequence of Star in STARKO2 cells (top) compared to wild-type mouse Star (bottom). Dashes represent nucleotide deletions. Amino acid sequence of wild-type STAR (top) compared to that of STARKO2 (bottom). Underlined amino acids represent sequence changes.

    Techniques Used: Biomarker Discovery, CRISPR, Western Blot, Quantitative RT-PCR, Sequencing

    Related Articles

    CRISPR:

    Article Title: Role of Constitutive STAR in Leydig Cells
    Article Snippet: .. Two sets of ss DNA oligonucleotides specifically targeting exon2 of Star were designed after using Synthego’s CRISPR single gRNA Design Tool ( https://www.synthego.com ). .. The ss oligonucleotides were annealed and cloned into the GeneArt ® CRISPR Nuclease Vector which contains an OFP reporter (Thermo Fisher Scientific, Waltham, MA, USA).



    Similar Products

    crispr single grna design tool  (Synthego Inc)
    90
    Synthego Inc crispr single grna design tool
    Screening and validation of <t>CRISPR/Cas9-mediated</t> STAR KO in MA-10 cells. ( A ) Western blot screening for STAR KO cell line following CRISPR/Cas9-mediated STAR KO and FACS. Samples in lane 5 (STARKO2) and lane 8 (STARKO1) show no STAR band and, therefore, are STAR KOs. ( B ) qRT-PCR analyses of STARKO1 and STARKO2 where Gapdh was used as the housekeeping gene. STAR 1, STAR 2, STAR 3, and STAR 4 are primers for various regions of the mouse Star gene. Data are shown as the mean ± SD ( n = 3). ** p < 0.01; *** p < 0.001. ( C ) BLAST DNA sequence of Star in STARKO1 cells (top) compared to wild-type mouse Star (bottom). Dashes represent nucleotide deletions. Amino acid sequence of wild-type STAR (top) compared to that of STARKO1 (bottom). Highlighted amino acids represent sequence changes. ( D ) BLAST DNA sequence of Star in STARKO2 cells (top) compared to wild-type mouse Star (bottom). Dashes represent nucleotide deletions. Amino acid sequence of wild-type STAR (top) compared to that of STARKO2 (bottom). Underlined amino acids represent sequence changes.
    Crispr Single Grna Design Tool, supplied by Synthego Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/crispr+single+grna+design+tool/crispr+single+grna+design+tool/pmc07922663-169-16-22
    Average 90 stars, based on 1 article reviews
    crispr single grna design tool - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Screening and validation of CRISPR/Cas9-mediated STAR KO in MA-10 cells. ( A ) Western blot screening for STAR KO cell line following CRISPR/Cas9-mediated STAR KO and FACS. Samples in lane 5 (STARKO2) and lane 8 (STARKO1) show no STAR band and, therefore, are STAR KOs. ( B ) qRT-PCR analyses of STARKO1 and STARKO2 where Gapdh was used as the housekeeping gene. STAR 1, STAR 2, STAR 3, and STAR 4 are primers for various regions of the mouse Star gene. Data are shown as the mean ± SD ( n = 3). ** p < 0.01; *** p < 0.001. ( C ) BLAST DNA sequence of Star in STARKO1 cells (top) compared to wild-type mouse Star (bottom). Dashes represent nucleotide deletions. Amino acid sequence of wild-type STAR (top) compared to that of STARKO1 (bottom). Highlighted amino acids represent sequence changes. ( D ) BLAST DNA sequence of Star in STARKO2 cells (top) compared to wild-type mouse Star (bottom). Dashes represent nucleotide deletions. Amino acid sequence of wild-type STAR (top) compared to that of STARKO2 (bottom). Underlined amino acids represent sequence changes.

    Journal: International Journal of Molecular Sciences

    Article Title: Role of Constitutive STAR in Leydig Cells

    doi: 10.3390/ijms22042021

    Figure Lengend Snippet: Screening and validation of CRISPR/Cas9-mediated STAR KO in MA-10 cells. ( A ) Western blot screening for STAR KO cell line following CRISPR/Cas9-mediated STAR KO and FACS. Samples in lane 5 (STARKO2) and lane 8 (STARKO1) show no STAR band and, therefore, are STAR KOs. ( B ) qRT-PCR analyses of STARKO1 and STARKO2 where Gapdh was used as the housekeeping gene. STAR 1, STAR 2, STAR 3, and STAR 4 are primers for various regions of the mouse Star gene. Data are shown as the mean ± SD ( n = 3). ** p < 0.01; *** p < 0.001. ( C ) BLAST DNA sequence of Star in STARKO1 cells (top) compared to wild-type mouse Star (bottom). Dashes represent nucleotide deletions. Amino acid sequence of wild-type STAR (top) compared to that of STARKO1 (bottom). Highlighted amino acids represent sequence changes. ( D ) BLAST DNA sequence of Star in STARKO2 cells (top) compared to wild-type mouse Star (bottom). Dashes represent nucleotide deletions. Amino acid sequence of wild-type STAR (top) compared to that of STARKO2 (bottom). Underlined amino acids represent sequence changes.

    Article Snippet: Two sets of ss DNA oligonucleotides specifically targeting exon2 of Star were designed after using Synthego’s CRISPR single gRNA Design Tool ( https://www.synthego.com ).

    Techniques: Biomarker Discovery, CRISPR, Western Blot, Quantitative RT-PCR, Sequencing